The following recommendations detail proper handling procedures for the dissociating enzyme and iCell Cardiomyocytes cultured for 7 days post-plating in 6-well cell culture plates.

We have optimized the dissociation for iCell Hepatocytes 2.0 using TrypLE Express Enzyme (Thermo Fisher Scientific, Cat. No. 12563011) or StemPro Accutase Cell Dissociation Reagent (Thermo …

The GBA gene encodes glucocerebrosidase (GCase), a lysosomal enzyme that metabolizes glucocerebroside into ceramide and glucose. The GBA N370S mutation is associated with aberrant α …

We have optimized the dissociation for iCell Hepatocytes 2.0 using TrypLE Express Enzyme (Thermo Fisher Scientific, Cat. No. 12563011) or StemPro Accutase Cell Dissociation Reagent (Thermo...

The following recommendations detail proper handling procedures for the dissociating enzyme and iCell Cardiomyocytes cultured for 7 days post-plating in 6-well cell culture plates.

(D) linear correlation between amount of protein lysate and enzyme activity. (E) CBE is a specific inhibitor of GCase. (F) All mutant lines showed decreased activity compared to AHN; (G) Screen for …

6. Gently mix by aspirating and dispensing the reaction 2 - 3 times. Briefly centrifuge to collect the contents at the bottom of the wells. 7. Incubate in a qRT-PCR thermal cycler at 37°C for 60 minutes …

Collecting iCell Cardiomyocytes from the 6-well Cell Culture Plate to excessive mechanical trituration. The following recommendations detail proper handling procedures for the dissociating enzyme and …

May 16, 2017 · Basal cytochrome P450 enzyme activities of CYP1A2, CYP2B6 and CYP3A4/5 were determined with probe substrates phenacetin, bupropion and midazolam, respectively, and analyzed …

* The cell culturing procedures described here were optimized using either TrypLE Express Enzyme (ThermoFisher Scientific, Cat. No. 12563) or StemPro Accutase Cell Dissociation Reagent …